Hotspot

trnS-GCU-trnG-GCC

LSC · 7211-8153 · 943 bp

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Pi 0.0085
MarkerSeek score 58.10
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
943
variable_sites
8
pi
0.0085
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA AGGGATCCCTTAACTATTAGGCT 50.0 43.5 58.0 57.9 1043-1047 bp 1.0 55.9
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC AGGGATCCCTTAACTATTAGGCT 50.0 43.5 58.0 57.9 1042-1046 bp 1.0 55.9
trnS-GCU-trnG-GCC_p3 ATCAACGGAACCGGAAAGAG AGGGATCCCTTAACTATTAGGCT 50.0 43.5 57.9 57.9 1107-1111 bp 1.0 55.1
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA GAATAGTTATTCCCCGAGGG 50.0 50.0 58.0 54.7 1059-1063 bp 1.0 54.3
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC GAATAGTTATTCCCCGAGGG 50.0 50.0 58.0 54.7 1058-1062 bp 1.0 54.3