Hotspot

psbI-trnS-GCU

LSC · 6989-7122 · 134 bp

Back to Moquilea

Pi 0.0224

MarkerSeek score 65.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 134
variable_sites: 3
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TTTATCTAATGACCCGGGGC	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.1	58.0	250-256 bp	1.0	73.4
psbI-trnS-GCU_p2	TTTATCTAATGACCCGGGGC	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.1	58.0	251-257 bp	1.0	73.4
psbI-trnS-GCU_p3	TTTATCTAATGACCCGGGGC	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	269-275 bp	1.0	72.5
psbI-trnS-GCU_p4	TTTATCTAATGACCCGGGGC	CATTGGGAGAGATGGCTGAG	50.0	55.0	57.1	57.7	270-276 bp	1.0	71.3
psbI-trnS-GCU_p5	TTTTATCTAATGACCCGGGGC	TGGACTAAAGCGTCGGATTG	47.6	50.0	57.8	58.0	251-257 bp	1.0	71.2