Hotspot

trnQ-UUG-psbK

LSC · 5853-6236 · 384 bp

Back to Moquilea

Pi 0.0156

MarkerSeek score 58.10

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 6
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GTTGCCAAAGCCGGTAATTT	TAGGCTTCGGGCAATTTTGT	45.0	45.0	57.6	58.1	591-606 bp	1.0	76.6
trnQ-UUG-psbK_p2	ATAGGTATACGCTGGGACGG	TAGGCTTCGGGCAATTTTGT	55.0	45.0	58.5	58.1	556-570 bp	1.0	76.5
trnQ-UUG-psbK_p3	GTTGCCAAAGCCGGTAATTT	GCTTCGGGCAATTTTGTGAA	45.0	45.0	57.6	57.9	588-603 bp	1.0	76.0
trnQ-UUG-psbK_p4	ATAGGTATACGCTGGGACGG	GCTTCGGGCAATTTTGTGAA	55.0	45.0	58.5	57.9	553-567 bp	1.0	76.0
trnQ-UUG-psbK_p5	GTTGCCAAAGCCGGTAATTT	GTAGGCTTCGGGCAATTTTG	45.0	50.0	57.6	57.7	592-607 bp	1.0	75.0