Hotspot

tRNA-Asn(GUU)-ndhF

SSC · 115497-116319 · 823 bp

Back to Monstera

Pi 0.0275

MarkerSeek score 77.20

Primer available yes

Label tRNA-Asn(GUU)-ndhF

Feature Metadata

label_name: tRNA-Asn(GUU)-ndhF
feature_type: igs
region: SSC
length_bp: 823
variable_sites: 21
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asn(GUU)-ndhF_p1	AGTCAGTTAACAGCCAACCG	GGGGCGAATCTCGTCTTATC	50.0	55.0	58.1	57.9	901-954 bp	1.0	84.3
tRNA-Asn(GUU)-ndhF_p2	ACGACCAGTCAGTTAACAGC	GGGGCGAATCTCGTCTTATC	50.0	55.0	57.8	57.9	907-960 bp	1.0	84.1
tRNA-Asn(GUU)-ndhF_p3	AGGATTCGAACCTACGACCA	GGGGCGAATCTCGTCTTATC	50.0	55.0	58.2	57.9	920-973 bp	1.0	84.0
tRNA-Asn(GUU)-ndhF_p4	GGATTCGAACCTACGACCAG	GGGGCGAATCTCGTCTTATC	55.0	55.0	57.8	57.9	919-972 bp	1.0	83.6
tRNA-Asn(GUU)-ndhF_p5	CCCCAAGTAGGATTCGAACC	GGGGCGAATCTCGTCTTATC	55.0	55.0	57.7	57.9	928-981 bp	1.0	82.6