Hotspot

rps19-rpl2

IRb · 89987-90045 · 59 bp

Back to Monstera

Pi 0.119

MarkerSeek score 76.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 59
variable_sites: 7
pi: 0.119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	ACCAATAAATGATTAGCTACAAAAGGA	CGTTTTATTCTTCGTCGCCG	29.6	50.0	57.5	57.9	136-149 bp	1.0	43.9
rps19-rpl2_p2	ACCAATAAATGATTAGCTACAAAAGG	CGTTTTATTCTTCGTCGCCG	30.8	50.0	56.3	57.9	136-149 bp	1.0	43.9
rps19-rpl2_p3	ACCAATAAATGATTAGCTACAAAAGGA	GTTATCCTGCGCTTGGAAGA	29.6	50.0	57.5	58.0	186-199 bp	1.0	42.9
rps19-rpl2_p4	ACCAATAAATGATTAGCTACAAAAGG	GTTATCCTGCGCTTGGAAGA	30.8	50.0	56.3	58.0	186-199 bp	1.0	42.9
rps19-rpl2_p5	ACCAATAAATGATTAGCTACAAAAGGA	TATCCTGCGCTTGGAAGAAG	29.6	50.0	57.5	57.7	184-197 bp	1.0	42.9