Hotspot

rps4-tRNA-Thr(UGU)

LSC · 49097-49642 · 546 bp

Back to Monstera

Pi 0.0278

MarkerSeek score 74.60

Primer available yes

Label rps4-tRNA-Thr(UGU)

Feature Metadata

label_name: rps4-tRNA-Thr(UGU)
feature_type: igs
region: LSC
length_bp: 546
variable_sites: 15
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-tRNA-Thr(UGU)_p1	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.6	57.9	674-744 bp	1.0	78.0
rps4-tRNA-Thr(UGU)_p2	GTTAGTCCTGGTAAAGCCCC	GATGGTCATCGGTTCGACTC	55.0	55.0	57.6	58.2	643-713 bp	1.0	77.9
rps4-tRNA-Thr(UGU)_p3	TTAGTCCTGGTAAAGCCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.7	57.9	673-743 bp	1.0	75.6
rps4-tRNA-Thr(UGU)_p4	TTAGTCCTGGTAAAGCCCCC	GATGGTCATCGGTTCGACTC	55.0	55.0	58.7	58.2	642-712 bp	1.0	75.5
rps4-tRNA-Thr(UGU)_p5	GTTAGTCCTGGTAAAGCCCC	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	57.6	57.5	682-752 bp	1.0	75.0