Hotspot

tRNA-Glu(UUC)-tRNA-Thr(GGU)

LSC · 33465-34400 · 936 bp

Back to Monstera

Pi 0.0178

MarkerSeek score 71.00

Primer available yes

Label tRNA-Glu(UUC)-tRNA-Thr(GGU)

Feature Metadata

label_name: tRNA-Glu(UUC)-tRNA-Thr(GGU)
feature_type: igs
region: LSC
length_bp: 936
variable_sites: 15
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	931-1023 bp	1.0	84.0
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	929-1021 bp	1.0	84.0
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	910-1002 bp	1.0	83.6
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	908-1000 bp	1.0	83.6
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p5	AGATGTCCTGAACCGCTAGA	AGTTTCGTGGAAAAAGCCCT	50.0	45.0	57.9	57.9	960-1052 bp	1.0	83.5