Hotspot

atpH-atpI

LSC · 14816-15705 · 890 bp

Back to Monstera

Pi 0.0102

MarkerSeek score 65.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 9
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TCACAAGCGGTATTCAAGCT	45.0	45.0	58.1	57.5	997-1000 bp	1.0	76.8
atpH-atpI_p2	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	973-976 bp	1.0	76.4
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	TCACAAGCGGTATTCAAGCT	50.0	45.0	57.8	57.5	1007-1010 bp	1.0	76.2
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	983-986 bp	1.0	75.9
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TCACAAGCGGTATTCAAGCT	50.0	45.0	57.8	57.5	1014-1017 bp	1.0	75.8