Hotspot

tRNA-Ser(GCU)-tRNA-Ser (CGA)

LSC · 9186-9969 · 784 bp

Back to Monstera

Pi 0.0174

MarkerSeek score 67.30

Primer available yes

Label tRNA-Ser(GCU)-tRNA-Ser (CGA)

Feature Metadata

label_name: tRNA-Ser(GCU)-tRNA-Ser (CGA)
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 13
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p1	ATTAGCAATCCGCCGCTTTA	AGGACGAATCACACTTTTACCA	45.0	40.9	58.0	57.7	861-905 bp	1.0	65.5
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p2	ATTAGCAATCCGCCGCTTTA	GGACGAATCACACTTTTACCAC	45.0	45.5	58.0	57.6	860-904 bp	1.0	65.1
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p3	AAAGCGGAGAGAGAGGGATT	AGGACGAATCACACTTTTACCA	50.0	40.9	58.1	57.7	913-957 bp	1.0	64.6
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p4	CAAGTGACCAGAGAAAGCGA	AGGACGAATCACACTTTTACCA	50.0	40.9	57.8	57.7	932-976 bp	1.0	64.3
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p5	TCAAGTGACCAGAGAAAGCG	AGGACGAATCACACTTTTACCA	50.0	40.9	57.8	57.7	933-977 bp	1.0	64.3