Hotspot

tRNA-Lys(UUU)-rps16

LSC · 4373-5037 · 665 bp

Back to Monstera

Pi 0.0105

MarkerSeek score 63.60

Primer available yes

Label tRNA-Lys(UUU)-rps16

Feature Metadata

label_name: tRNA-Lys(UUU)-rps16
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 7
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Lys(UUU)-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAGGAGCTCAACCCACAAG	50.0	50.0	58.2	58.0	763-767 bp	1.0	79.3
tRNA-Lys(UUU)-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAGGAGCTCAACCCACAAG	50.0	50.0	58.2	58.0	762-766 bp	1.0	79.3
tRNA-Lys(UUU)-rps16_p3	AAAGCCGAGTACTCTACCGT	AGCTCAACCCACAAGAACTG	50.0	50.0	58.2	58.0	758-762 bp	1.0	79.2
tRNA-Lys(UUU)-rps16_p4	AAGCCGAGTACTCTACCGTT	AGCTCAACCCACAAGAACTG	50.0	50.0	58.2	58.0	757-761 bp	1.0	79.2
tRNA-Lys(UUU)-rps16_p5	CCGCACTTAAAAGCCGAGTA	AAAGGAGCTCAACCCACAAG	50.0	50.0	58.3	58.0	772-776 bp	1.0	78.4