Hotspot

A3228_gp07-A3228_gp06

Genome · 18170-19447 · 1278 bp

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Pi 0.226

MarkerSeek score 84.50

Primer available yes

Label A3228_gp07-A3228_gp06

Feature Metadata

label_name: A3228_gp07-A3228_gp06
feature_type: igs
region: Genome
length_bp: 1278
variable_sites: 415
pi: 0.226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gp07-A3228_gp06_p1	CGGGAATCTATGCCTTGCTT	CCCATCGATATCTCCCTGGT	50.0	55.0	58.0	58.1	1966-2017 bp	0.666667	72.5
A3228_gp07-A3228_gp06_p2	GGGGATGAGGATGAGGATGA	CCCATCGATATCTCCCTGGT	55.0	55.0	58.0	58.1	2339 bp	0.333333	52.6
A3228_gp07-A3228_gp06_p3	CGGGAATCTATGCCTTGCTT	CAGTTGATTCCTTGCACCCT	50.0	50.0	58.0	58.1	1958 bp	0.333333	52.4
A3228_gp07-A3228_gp06_p4	CGGGAATCTATGCCTTGCTT	TCAGTTGATTCCTTGCACCC	50.0	50.0	58.0	58.1	1959 bp	0.333333	52.4
A3228_gp07-A3228_gp06_p5	GGGGATGAGGATGAGGATGA	CAGTTGATTCCTTGCACCCT	55.0	50.0	58.0	58.1	2280 bp	0.333333	52.4