Hotspot

A3228_gt05-A3228_gp09

Genome · 11668-12231 · 564 bp

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Pi 0.351
MarkerSeek score 78.80
Primer available yes
Label A3228_gt05-A3228_gp09

Feature Metadata

label_name
A3228_gt05-A3228_gp09
feature_type
igs
region
Genome
length_bp
564
variable_sites
233
pi
0.351
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
A3228_gt05-A3228_gp09_p1 GTAGCTCGCAAGGCTCATAA AGGCTTTTGTCCATCCCTTG 50.0 50.0 57.8 58.1 1645 bp 0.333333 51.0
A3228_gt05-A3228_gp09_p2 TAGCTCGCAAGGCTCATAAC AGGCTTTTGTCCATCCCTTG 50.0 50.0 57.8 58.1 1644 bp 0.333333 51.0
A3228_gt05-A3228_gp09_p3 GTAGCTCGCAAGGCTCATAA TGAAACGGGGCCCTCTATAA 50.0 50.0 57.8 58.1 1241 bp 0.333333 50.6
A3228_gt05-A3228_gp09_p4 TAGCTCGCAAGGCTCATAAC TGAAACGGGGCCCTCTATAA 50.0 50.0 57.8 58.1 1240 bp 0.333333 50.6
A3228_gt05-A3228_gp09_p5 TAGCTCGCAAGGCTCATAAC TTGAAACGGGGCCCTCTATA 50.0 50.0 57.8 58.1 1241 bp 0.333333 50.6