Hotspot

A3228_gp21-A3228_gp20

Genome · 4137-5307 · 1171 bp

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Pi 0.153

MarkerSeek score 81.50

Primer available yes

Label A3228_gp21-A3228_gp20

Feature Metadata

label_name: A3228_gp21-A3228_gp20
feature_type: igs
region: Genome
length_bp: 1171
variable_sites: 246
pi: 0.153
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gp21-A3228_gp20_p1	CGAATGGATCAGAAAGGGCA	TCGTCGATTGCTAGCTCCTA	50.0	50.0	58.0	58.0	1903 bp	0.333333	52.7
A3228_gp21-A3228_gp20_p2	GGTTTGCACACCCAAAAGAC	TCGTCGATTGCTAGCTCCTA	50.0	50.0	58.1	58.0	2381 bp	0.333333	52.5
A3228_gp21-A3228_gp20_p3	CGAATGGATCAGAAAGGGCA	AACCCATGCGTGCTTCTTTA	50.0	45.0	58.0	58.1	1563 bp	0.333333	52.2
A3228_gp21-A3228_gp20_p4	CGAATGGATCAGAAAGGGCA	GGTTTAACAACGGCTACCCA	50.0	50.0	58.0	58.1	1606 bp	0.333333	52.2
A3228_gp21-A3228_gp20_p5	CGAATGGATCAGAAAGGGCA	TGGTTTAACAACGGCTACCC	50.0	50.0	58.0	58.1	1607 bp	0.333333	52.2