Hotspot

A3228_gr04

Genome · 14576-16049 · 1474 bp

Back to Monotropa

Pi 0.0927

MarkerSeek score 79.20

Primer available yes

Label A3228_gr04

Feature Metadata

label_name: A3228_gr04
feature_type: rRNA
region: Genome
length_bp: 1474
variable_sites: 167
pi: 0.0927
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gr04_p1	AAAGTGAAGACCGGGCTTTT	AGCTTTGCATTGGAATCCCA	45.0	45.0	57.9	58.1	2539 bp	0.333333	52.3
A3228_gr04_p2	AAGTGAAGACCGGGCTTTTT	AGCTTTGCATTGGAATCCCA	45.0	45.0	57.9	58.1	2538 bp	0.333333	52.3
A3228_gr04_p3	AAGTGAAGACCGGGCTTTTT	AGAGAAACCCCATCCCTCTC	45.0	55.0	57.9	58.1	2376 bp	0.333333	51.9
A3228_gr04_p4	AAGTGAAGACCGGGCTTTTT	GAGAAACCCCATCCCTCTCT	45.0	55.0	57.9	58.1	2375 bp	0.333333	51.9
A3228_gr04_p5	AAGTGAAGACCGGGCTTTTT	GAGAGAAACCCCATCCCTCT	45.0	55.0	57.9	58.1	2377 bp	0.333333	51.9