Hotspot

A3228_gp09

Genome · 12232-12843 · 612 bp

Back to Monotropa

Pi 0.253

MarkerSeek score 80.40

Primer available yes

Label A3228_gp09

Feature Metadata

label_name: A3228_gp09
feature_type: gene
region: Genome
length_bp: 612
variable_sites: 229
pi: 0.253
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
A3228_gp09_p1	GGCTCAGGATTGCCCTTTTA	AATCCAGCTTTTCCTTGCCA	50.0	45.0	57.9	58.0	1396 bp	0.333333	52.1
A3228_gp09_p2	GGCTCAGGATTGCCCTTTTA	ACTAGATCCGGTTACCCCTG	50.0	55.0	57.9	57.9	1672 bp	0.333333	51.5
A3228_gp09_p3	GGCTCAGGATTGCCCTTTTA	ATTCCTCGGTGGGGATTGTA	50.0	50.0	57.9	58.1	1534 bp	0.333333	51.3
A3228_gp09_p4	GGCTCAGGATTGCCCTTTTA	TATTCCTCGGTGGGGATTGT	50.0	50.0	57.9	58.1	1535 bp	0.333333	51.3
A3228_gp09_p5	GGCTCAGGATTGCCCTTTTA	TAGCTCTCGCTCTTCCAGTT	50.0	50.0	57.9	58.2	1589 bp	0.333333	50.9