Hotspot

ycf1

IRa · 134126-141721 · 7596 bp

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Pi 0.0734

MarkerSeek score 72.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 7596
variable_sites: 537
pi: 0.0734
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGATCCTTTTCGTCCTGCTT	AAATGCAGTCAGCCATAGGG	50.0	50.0	57.9	57.9	1557 bp	0.5	58.6
ycf1_p2	GGATCCTTTTCGTCCTGCTT	TTACCGCTCACAGTTAAGGC	50.0	50.0	57.9	57.9	950 bp	0.5	58.4
ycf1_p3	TGGATTAGTGTAGTCGGGCA	AAATGCAGTCAGCCATAGGG	50.0	50.0	58.1	57.9	1179 bp	0.5	58.3
ycf1_p4	GGATCCTTTTCGTCCTGCTT	ATCGAACGTAAGCCGAAGAC	50.0	50.0	57.9	58.1	911 bp	0.5	58.3
ycf1_p5	TGGATTAGTGTAGTCGGGCA	TTACCGCTCACAGTTAAGGC	50.0	50.0	58.1	57.9	572 bp	0.5	58.1