Hotspot

rps18

LSC · 59215-59679 · 465 bp

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Pi 0.153

MarkerSeek score 78.90

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 465
variable_sites: 63
pi: 0.153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	GCGACGTAGAACAAAAAGGC	AGTTAGTGACGTGGGAAGGA	50.0	50.0	58.1	58.0	1719-1743 bp	1.0	91.5
rps18_p2	GTCGTTTGTGGATCACTCGA	AGTTAGTGACGTGGGAAGGA	50.0	50.0	57.9	58.0	1594-1618 bp	1.0	91.5
rps18_p3	TCGTCGTTTGTGGATCACTC	AGTTAGTGACGTGGGAAGGA	50.0	50.0	57.9	58.0	1596-1620 bp	1.0	91.5
rps18_p4	GCGACGTAGAACAAAAAGGC	TAGGTTCATGGCCAAGGGTA	50.0	50.0	58.1	58.0	1313-1370 bp	1.0	91.4
rps18_p5	ATTGACTCGGAAGGGAGACA	AGTTAGTGACGTGGGAAGGA	50.0	50.0	58.1	58.0	1858 bp	0.5	59.5