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ORF100

LSC · 52238-52540 · 303 bp

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Pi 0.153

MarkerSeek score 63.80

Primer available yes

Label ORF100

Feature Metadata

label_name: ORF100
feature_type: gene
region: LSC
length_bp: 303
variable_sites: 23
pi: 0.153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.50
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF100_p1	CACGTGTCAAAGTCCTAGCA	GTTGAGCACCCCTTTCAAGA	50.0	50.0	57.9	58.0	1178-1349 bp	1.0	88.0
ORF100_p2	ACACGTGTCAAAGTCCTAGC	GTTGAGCACCCCTTTCAAGA	50.0	50.0	57.8	58.0	1179-1350 bp	1.0	88.0
ORF100_p3	ACGTGTCAAAGTCCTAGCAC	GTTGAGCACCCCTTTCAAGA	50.0	50.0	57.8	58.0	1177-1348 bp	1.0	88.0
ORF100_p4	CACGTGTCAAAGTCCTAGCA	AAAACCCACTTTCCGAAGCT	50.0	45.0	57.9	57.9	1276 bp	0.5	58.3
ORF100_p5	ACGTGTCAAAGTCCTAGCAC	AAAACCCACTTTCCGAAGCT	50.0	45.0	57.8	57.9	1275 bp	0.5	58.3