Hotspot

ndhD-ccsA

SSC · 122521-122790 · 270 bp

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Pi 0.0613
MarkerSeek score 75.80
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
270
variable_sites
16
pi
0.0613
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 CCGGATTTTGTTCTTTCACTCTC AGGGCTACATAGTTATGGTTCA 43.5 40.9 58.0 56.3 367-376 bp 1.0 45.4
ndhD-ccsA_p2 TCCGGATTTTGTTCTTTCACTC AGGGCTACATAGTTATGGTTCA 40.9 40.9 56.9 56.3 368-377 bp 1.0 45.4
ndhD-ccsA_p3 CCGGATTTTGTTCTTTCACTCT AGGGCTACATAGTTATGGTTCA 40.9 40.9 56.9 56.3 367-376 bp 1.0 45.4
ndhD-ccsA_p4 TCCGGATTTTGTTCTTTCACT AGGGCTACATAGTTATGGTTCA 38.1 40.9 55.7 56.3 368-377 bp 1.0 45.4
ndhD-ccsA_p5 TCCGGATTTTGTTCTTTCACTCT AGGGCTACATAGTTATGGTTCA 39.1 40.9 58.3 56.3 368-377 bp 1.0 45.4