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rpl23-trnI (CAU)

IRb · 82998-83516 · 519 bp

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Pi 0.0938

MarkerSeek score 79.70

Primer available yes

Label rpl23-trnI (CAU)

Feature Metadata

label_name: rpl23-trnI (CAU)
feature_type: igs
region: IRb
length_bp: 519
variable_sites: 47
pi: 0.0938
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnI (CAU)_p1	TCTTCTAGCCTTTCCCGGAA	CCTGTTAAGCATCCATGGCT	50.0	50.0	58.1	57.9	914-1061 bp	1.0	86.4
rpl23-trnI (CAU)_p2	CGGTATAGACGCTTATGGCC	CCTGTTAAGCATCCATGGCT	55.0	50.0	58.0	57.9	1198-1340 bp	1.0	85.5
rpl23-trnI (CAU)_p3	ACTGTCTACGGTTCCATTGC	CCTGTTAAGCATCCATGGCT	50.0	50.0	57.9	57.9	1067-1209 bp	1.0	85.5
rpl23-trnI (CAU)_p4	TTATGGCCTCCCCCTCTATG	CCTGTTAAGCATCCATGGCT	55.0	50.0	58.0	57.9	1186-1328 bp	1.0	85.3
rpl23-trnI (CAU)_p5	ACCACAAAGATGCTGTCCAG	CCTGTTAAGCATCCATGGCT	50.0	50.0	58.1	57.9	1130 bp	0.5	58.7