Hotspot

trnK (UUU)-ORF148

LSC · 56629-56807 · 179 bp

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Pi 0.115
MarkerSeek score 77.20
Primer available yes
Label trnK (UUU)-ORF148

Feature Metadata

label_name
trnK (UUU)-ORF148
feature_type
igs
region
LSC
length_bp
179
variable_sites
20
pi
0.115
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK (UUU)-ORF148_p1 CTAGTTCCGGGTTCGAATCC ACCGTGAGATTCAAATCATAGA 55.0 36.4 57.8 55.0 235-236 bp 1.0 48.5
trnK (UUU)-ORF148_p2 CTAGTTCCGGGTTCGAATCC ACCGTGAGATTCAAATCATAGAT 55.0 34.8 57.8 55.5 235-236 bp 1.0 48.5
trnK (UUU)-ORF148_p3 CTAGTTCCGGGTTCGAATCC ACCGTGAGATTCAAATCATAG 55.0 38.1 57.8 53.4 235-236 bp 1.0 48.5
trnK (UUU)-ORF148_p4 ATCCGACTAGTTCCGGGTTC ACCGTGAGATTCAAATCATAGA 55.0 36.4 58.9 55.0 241-242 bp 1.0 48.3
trnK (UUU)-ORF148_p5 CTTCATCCGACTAGTTCCGG ACCGTGAGATTCAAATCATAGA 55.0 36.4 57.5 55.0 245-246 bp 1.0 48.1