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psbK-ORF100

LSC · 51526-52237 · 712 bp

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Pi 0.0651

MarkerSeek score 77.30

Primer available yes

Label psbK-ORF100

Feature Metadata

label_name: psbK-ORF100
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 45
pi: 0.0651
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-ORF100_p1	CTCGTAGCAAGGGCAGAATT	AAGTCGCACGTTGCTTTCTA	50.0	45.0	58.0	58.1	1318-1489 bp	1.0	87.6
psbK-ORF100_p2	CTCGTAGCAAGGGCAGAATT	TAAGTCGCACGTTGCTTTCT	50.0	45.0	58.0	58.1	1319-1490 bp	1.0	87.6
psbK-ORF100_p3	CTCGTAGCAAGGGCAGAATT	GATTCGCGCCAAAATAGTGG	50.0	50.0	58.0	57.9	962 bp	0.5	58.9
psbK-ORF100_p4	CTTACAGCAGCTTGCCAAAC	GATTCGCGCCAAAATAGTGG	50.0	50.0	57.9	57.9	1082 bp	0.5	58.7
psbK-ORF100_p5	CTCGTAGCAAGGGCAGAATT	CGATTCGCGTCAAAATCGTT	50.0	45.0	58.0	57.9	910 bp	0.5	58.6