Hotspot

atpB-rbcL

LSC · 47780-48565 · 786 bp

Back to Monopsis

Pi 0.028

MarkerSeek score 74.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 22
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACCCCAGAACCAGAAG	CGTCTCTGTTTGTGGTGACA	55.0	50.0	58.1	58.1	846 bp	1.0	80.8
atpB-rbcL_p2	AGGACCGGACCAATGATTTG	CGTCTCTGTTTGTGGTGACA	50.0	50.0	57.9	58.1	899 bp	1.0	80.4
atpB-rbcL_p3	ACATCTAGGACCGGACCAAT	CGTCTCTGTTTGTGGTGACA	50.0	50.0	57.8	58.1	905 bp	1.0	80.0
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	TCGTCTCTGTTTGTGGTGAC	55.0	50.0	58.1	57.8	847 bp	1.0	79.6
atpB-rbcL_p5	AGGACCGGACCAATGATTTG	TCGTCTCTGTTTGTGGTGAC	50.0	50.0	57.9	57.8	900 bp	1.0	79.2