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trnM (CAU)-atpE

LSC · 45669-45884 · 216 bp

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Pi 0.107

MarkerSeek score 78.80

Primer available yes

Label trnM (CAU)-atpE

Feature Metadata

label_name: trnM (CAU)-atpE
feature_type: igs
region: LSC
length_bp: 216
variable_sites: 23
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM (CAU)-atpE_p1	TATTGCTTTCATACGGCGGG	GTCGAGGCTATCAATGCGAT	50.0	50.0	58.4	57.9	291-292 bp	1.0	82.7
trnM (CAU)-atpE_p2	GTATTGCTTTCATACGGCGG	GTCGAGGCTATCAATGCGAT	50.0	50.0	57.3	57.9	292-293 bp	1.0	80.7
trnM (CAU)-atpE_p3	GCGGGAGTCATTGGTTCAAA	GTCGAGGCTATCAATGCGAT	50.0	50.0	58.8	57.9	276-277 bp	1.0	80.3
trnM (CAU)-atpE_p4	CTTTCATACGGCGGGAGTC	GTCGAGGCTATCAATGCGAT	57.9	50.0	58.0	57.9	286-287 bp	1.0	77.9
trnM (CAU)-atpE_p5	TATTGCTTTCATACGGCGGG	CTCTTCGACGAGCTAGGACA	50.0	55.0	58.4	58.6	314-315 bp	1.0	77.8