Hotspot

tufA

Genome · 66743-68072 · 1330 bp

Back to Monomorphina

Pi 0.169

MarkerSeek score 82.90

Primer available yes

Label tufA

Feature Metadata

label_name: tufA
feature_type: gene
region: Genome
length_bp: 1330
variable_sites: 208
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tufA_p1	GCACGTAATCGACCAGGAAA	GAAAACCTGTTGCACCAAGT	50.0	45.0	58.3	57.0	1801 bp	0.5	50.1
tufA_p2	GCACGTAATCGACCAGGAAA	ACCTGTTGCACCAAGTAAGA	50.0	45.0	58.3	57.0	1797 bp	0.5	49.6
tufA_p3	GCACGTAATCGACCAGGAAA	AGAAAACCTGTTGCACCAAG	50.0	45.0	58.3	56.7	1802 bp	0.5	47.7
tufA_p4	GCACGTAATCGACCAGGAAA	AACCTGTTGCACCAAGTAAGA	50.0	42.9	58.3	57.7	1798 bp	0.5	47.4
tufA_p5	AAATCCGCACGTAATCGACC	GAAAACCTGTTGCACCAAGT	50.0	45.0	58.7	57.0	1807 bp	0.5	46.6