Hotspot

rps3

Genome · 53890-55034 · 1145 bp

Back to Monomorphina

Pi 0.298

MarkerSeek score 82.80

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 1145
variable_sites: 328
pi: 0.298
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CGAAGACCTACCTTGAGCAC	GAAGCGTTTTCGTCCACATG	55.0	50.0	58.0	58.0	2015 bp	0.5	59.6
rps3_p2	CGAAGACCTACCTTGAGCAC	TTCGCCGAATCTTGGATCAG	55.0	50.0	58.0	58.1	2211 bp	0.5	59.5
rps3_p3	TAACCTCCACAGCCTCTTCA	GAAGCGTTTTCGTCCACATG	50.0	50.0	58.0	58.0	2184 bp	0.5	59.4
rps3_p4	CACATAACCTCCACAGCCTC	GAAGCGTTTTCGTCCACATG	55.0	50.0	58.0	58.0	2188 bp	0.5	59.4
rps3_p5	TAACCTCCACAGCCTCTTCA	TTCGCCGAATCTTGGATCAG	50.0	50.0	58.0	58.1	2380 bp	0.5	59.3