Hotspot

rpl16

Genome · 50804-51761 · 958 bp

Back to Monomorphina

Pi 0.278

MarkerSeek score 83.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: Genome
length_bp: 958
variable_sites: 252
pi: 0.278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGCAATAGGACCAAACACCC	GCTGGTTGGAGGTTGATTCT	50.0	50.0	58.1	57.8	2255 bp	0.5	57.7
rpl16_p2	GGCGCGTACAACATTTGATC	GCTGGTTGGAGGTTGATTCT	50.0	50.0	58.2	57.8	2024 bp	0.5	56.8
rpl16_p3	ATCCCTCAATTCACGTGCAA	GCTGGTTGGAGGTTGATTCT	45.0	50.0	57.8	57.8	2270 bp	0.5	56.8
rpl16_p4	TCCCTCAATTCACGTGCAAT	GCTGGTTGGAGGTTGATTCT	45.0	50.0	57.8	57.8	2269 bp	0.5	56.8
rpl16_p5	GCAATAGGACCAAACACCCT	GCTGGTTGGAGGTTGATTCT	50.0	50.0	57.8	57.8	2254 bp	0.5	56.6