Hotspot

orf631

Genome · 5503-7398 · 1896 bp

Back to Monomorphina

Pi 0.395

MarkerSeek score 82.70

Primer available yes

Label orf631

Feature Metadata

label_name: orf631
feature_type: gene
region: Genome
length_bp: 1896
variable_sites: 713
pi: 0.395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf631_p1	GCAATGTGCGTTTTAGCTTG	GACTCTTGATCACGACCACC	45.0	55.0	56.9	58.0	2163 bp	0.5	51.0
orf631_p2	GCAATGTGCGTTTTAGCTTG	AGACCACCAAGCAAATCCTG	45.0	50.0	56.9	58.1	2185 bp	0.5	50.4
orf631_p3	GCAATGTGCGTTTTAGCTTG	CAGACCACCAAGCAAATCCT	45.0	50.0	56.9	58.1	2186 bp	0.5	50.4
orf631_p4	GCAATGTGCGTTTTAGCTTG	ACCACCAAGCAAATCCTGTT	45.0	45.0	56.9	57.9	2183 bp	0.5	50.4
orf631_p5	GCAATGTGCGTTTTAGCTTG	TCCAGACCACCAAGCAAATC	45.0	50.0	56.9	58.1	2188 bp	0.5	50.3