Hotspot

orf240

Genome · 52989-53813 · 825 bp

Back to Monomorphina

Pi 0.42

MarkerSeek score 83.20

Primer available yes

Label orf240

Feature Metadata

label_name: orf240
feature_type: gene
region: Genome
length_bp: 825
variable_sites: 325
pi: 0.42
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf240_p1	AGAATCAACCTCCAACCAGC	AGAGAAGGACAAGTGCCTCT	50.0	50.0	57.8	58.0	1645 bp	0.5	58.2
orf240_p2	AGAATCAACCTCCAACCAGC	GAAGGACAAGTGCCTCTTCA	50.0	50.0	57.8	57.4	1642 bp	0.5	53.9
orf240_p3	CAACCTCCAACCAGCGAAAT	AGAGAAGGACAAGTGCCTCT	50.0	50.0	58.8	58.0	1640 bp	0.5	53.8
orf240_p4	AGAATCAACCTCCAACCAGC	CGGAAATCGCTAGGACAGAA	50.0	50.0	57.8	57.4	1671 bp	0.5	53.7
orf240_p5	ACCTCCAACCAGCGAAATAA	AGAGAAGGACAAGTGCCTCT	45.0	50.0	57.1	58.0	1638 bp	0.5	52.8