Hotspot

atpF

Genome · 43936-44803 · 868 bp

Back to Monomorphina

Pi 0.315

MarkerSeek score 83.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 868
variable_sites: 260
pi: 0.315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGATCTTTTTCTGAAGAGTCGG	TCACAAGTGGTATTCAGGCG	45.5	50.0	57.2	57.9	2147 bp	0.5	36.6
atpF_p2	CGATCTTTTTCTGAAGAGTCGG	GTACTGCTGCAGGTAAAGCT	45.5	50.0	57.2	57.9	1795 bp	0.5	36.5
atpF_p3	CGATCTTTTTCTGAAGAGTCGG	GTCTTGCTCGTCAACCTGAA	45.5	50.0	57.2	58.1	1768 bp	0.5	36.3
atpF_p4	CGATCTTTTTCTGAAGAGTCGG	TGAAGGTCTTGCTCGTCAAC	45.5	50.0	57.2	58.1	1773 bp	0.5	36.3
atpF_p5	CGATCTTTTTCTGAAGAGTCGG	TCGTCAACCTGAAGCTGAAG	45.5	50.0	57.2	57.8	1761 bp	0.5	36.1