Hotspot

atpE

Genome · 32227-33284 · 1058 bp

Back to Monomorphina

Pi 0.321

MarkerSeek score 83.50

Primer available yes

Label atpE

Feature Metadata

label_name: atpE
feature_type: gene
region: Genome
length_bp: 1058
variable_sites: 322
pi: 0.321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE_p1	ACAAGCAGGTTCCGAAGTTT	TCCGGCTTTAAATCCAGCTC	45.0	50.0	57.9	58.0	2348 bp	0.5	59.3
atpE_p2	ACAAGCAGGTTCCGAAGTTT	CGGCTTTAAATCCAGCTCCT	45.0	50.0	57.9	58.0	2346 bp	0.5	59.2
atpE_p3	ACAAGCAGGTTCCGAAGTTT	CTCCGGCTTTAAATCCAGCT	45.0	50.0	57.9	58.0	2349 bp	0.5	59.2
atpE_p4	TCGTAGTTTGGTCGCGATTT	TCCGGCTTTAAATCCAGCTC	45.0	50.0	57.9	58.0	1694 bp	0.5	59.1
atpE_p5	TCGTAGTTTGGTCGCGATTT	CGGCTTTAAATCCAGCTCCT	45.0	50.0	57.9	58.0	1692 bp	0.5	59.1