Hotspot

ndhF

SSC · 119816-122050 · 2235 bp

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Pi 0.0125

MarkerSeek score 57.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 156
pi: 0.0125
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTGCTTTGGGACGTTTACCA	TGCTCGAAAGGAATCGTTCT	45.0	45.0	57.9	57.3	2944-2995 bp	0.92	79.1
ndhF_p2	GGGACGTTTACCAGTGTCAG	TGCTCGAAAGGAATCGTTCT	55.0	45.0	58.2	57.3	2937-2988 bp	0.92	77.9
ndhF_p3	TTGGGACGTTTACCAGTGTC	TGCTCGAAAGGAATCGTTCT	50.0	45.0	57.8	57.3	2939-2990 bp	0.92	77.6
ndhF_p4	TTGCTTTGGGACGTTTACCA	TGCTCGAAAGGAATCGTTCTT	45.0	42.9	57.9	58.0	2944-2995 bp	0.92	76.7
ndhF_p5	TTGCTTTGGGACGTTTACCA	TTGCTCGAAAGGAATCGTTCT	45.0	42.9	57.9	58.0	2945-2996 bp	0.92	76.7