Hotspot

ndhE-ndhG

SSC · 127322-127503 · 182 bp

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Pi 0.0361

MarkerSeek score 72.40

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 182
variable_sites: 28
pi: 0.0361
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCGGCTCGTAATCAATCCA	ATTACCGTAGCTCGCCAGTA	45.0	50.0	57.0	58.3	272-281 bp	0.92	76.5
ndhE-ndhG_p2	TTCGGCTCGTAATCAATCCA	AGGTGCAATTACCGTAGCTC	45.0	50.0	57.0	57.7	279-288 bp	0.92	76.3
ndhE-ndhG_p3	TTCGGCTCGTAATCAATCCA	TTACCGTAGCTCGCCAGTAA	45.0	50.0	57.0	58.5	271-280 bp	0.92	74.7
ndhE-ndhG_p4	TCGGCTCGTAATCAATCCAT	ATTACCGTAGCTCGCCAGTA	45.0	50.0	56.8	58.3	271-280 bp	0.92	74.7
ndhE-ndhG_p5	TCGGCTCGTAATCAATCCAT	AGGTGCAATTACCGTAGCTC	45.0	50.0	56.8	57.7	278-287 bp	0.92	74.6