Hotspot

ccsA-ndhD

SSC · 124574-124900 · 327 bp

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Pi 0.0339
MarkerSeek score 72.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
327
variable_sites
61
pi
0.0339
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GGACTACACAGTTATGGCTCA TCAGTTGATAAGGTTGAAGCT 47.6 38.1 57.1 54.9 408-434 bp 1.0 50.1
ccsA-ndhD_p2 GGACTACACAGTTATGGCTCA TTCAGTTGATAAGGTTGAAGCT 47.6 36.4 57.1 55.6 409-435 bp 1.0 50.1
ccsA-ndhD_p3 AGGACTACACAGTTATGGCTC TCAGTTGATAAGGTTGAAGCT 47.6 38.1 56.8 54.9 409-435 bp 1.0 50.1
ccsA-ndhD_p4 GGACTACACAGTTATGGCTCA TTTCAGTTGATAAGGTTGAAGCT 47.6 34.8 57.1 56.4 410-436 bp 1.0 50.1
ccsA-ndhD_p5 AGGACTACACAGTTATGGCTC TTCAGTTGATAAGGTTGAAGCT 47.6 36.4 56.8 55.6 410-436 bp 1.0 50.1