Hotspot

rps19-rpl2

IRb · 82898-82992 · 95 bp

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Pi 0.0537

MarkerSeek score 75.10

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 95
variable_sites: 23
pi: 0.0537
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TTTGCTACAAACGGATTTTT	TTGATTCTTCGTCGCCGTAG	30.0	50.0	52.1	58.1	163-185 bp	1.0	52.0
rps19-rpl2_p2	TTTGCTACAAACGGATTTTT	TTGGGGTTATCCTGCACTTG	30.0	50.0	52.1	57.8	221-243 bp	1.0	52.0
rps19-rpl2_p3	TTTGCTACAAACGGATTTTT	GCAACTCCTTGGGGTTATCC	30.0	55.0	52.1	58.2	229-251 bp	1.0	52.0
rps19-rpl2_p4	TTTGCTACAAACGGATTTTT	ATTTGATTCTTCGTCGCCGT	30.0	45.0	52.1	58.3	165-187 bp	1.0	52.0
rps19-rpl2_p5	TTTGCTACAAACGGATTTTT	TTTGATTCTTCGTCGCCGTA	30.0	45.0	52.1	57.6	164-186 bp	1.0	52.0