Hotspot

ycf4-cemA

LSC · 60709-61334 · 626 bp

Back to Monnina

Pi 0.0177

MarkerSeek score 75.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 50
pi: 0.0177
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GACTCGTGCCGATGAGAATT	AGATCCACCAGGGCAAAAAG	50.0	50.0	58.1	58.1	775-809 bp	1.0	86.5
ycf4-cemA_p2	GACTCGTGCCGATGAGAATT	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.1	58.1	776-810 bp	1.0	86.5
ycf4-cemA_p3	GACTCGTGCCGATGAGAATT	GATCCACCAGGGCAAAAAGA	50.0	50.0	58.1	58.1	774-808 bp	1.0	86.5
ycf4-cemA_p4	GAGCCATTCCTTTGACTCGT	AGATCCACCAGGGCAAAAAG	50.0	50.0	57.9	58.1	788-822 bp	1.0	86.4
ycf4-cemA_p5	GAGCCATTCCTTTGACTCGT	GATCCACCAGGGCAAAAAGA	50.0	50.0	57.9	58.1	787-821 bp	1.0	86.3