Hotspot

trnT-UGU-accD

LSC · 56299-57876 · 1578 bp

Back to Monnina

Pi 0.016

MarkerSeek score 67.50

Primer available yes

Label trnT-UGU-accD

Feature Metadata

label_name: trnT-UGU-accD
feature_type: igs
region: LSC
length_bp: 1578
variable_sites: 114
pi: 0.016
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-accD_p1	CTAACCTCTGAGCTAAGCGG	GACTATTAAGATCTTTGGTCATAGT	55.0	32.0	57.5	54.2	1263-1633 bp	1.0	49.8
trnT-UGU-accD_p2	GCGATGCTCTAACCTCTGAG	GACTATTAAGATCTTTGGTCATAGT	55.0	32.0	57.9	54.2	1271-1641 bp	1.0	49.7
trnT-UGU-accD_p3	GCGATGCTCTAACCTCTGAG	ACTATTAAGATCTTTGGTCATAGT	55.0	29.2	57.9	53.0	1270-1640 bp	1.0	49.7
trnT-UGU-accD_p4	ATGCGATGCTCTAACCTCTG	GACTATTAAGATCTTTGGTCATAGT	50.0	32.0	57.5	54.2	1273-1643 bp	1.0	49.7
trnT-UGU-accD_p5	TGCGATGCTCTAACCTCTGA	GACTATTAAGATCTTTGGTCATAGT	50.0	32.0	58.5	54.2	1272-1642 bp	1.0	49.7