Hotspot

rps4-trnT-UGU

LSC · 55651-56225 · 575 bp

Back to Monnina

Pi 0.0159

MarkerSeek score 74.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 575
variable_sites: 51
pi: 0.0159
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCAGACGGCGAATTCTTTTG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.8	57.9	670-737 bp	1.0	85.0
rps4-trnT-UGU_p2	CCAGACGGCGAATTCTTTTG	CCTATGCGTGCGAGATACAA	50.0	50.0	57.8	57.9	707-774 bp	1.0	84.8
rps4-trnT-UGU_p3	CCAGACGGCGAATTCTTTTG	TTGAATTCCTATGCGTGCGA	50.0	45.0	57.8	58.0	714-781 bp	0.96	84.0
rps4-trnT-UGU_p4	TTCTTTTGAAACGCGGACCT	TTGAATTCCTATGCGTGCGA	45.0	45.0	58.3	58.0	702-769 bp	0.96	83.4
rps4-trnT-UGU_p5	CAGAGCGCGGTTGATTTCTA	TTGAATTCCTATGCGTGCGA	50.0	45.0	58.4	58.0	783-850 bp	0.96	82.6