Hotspot

atpF-atpH

LSC · 21499-21923 · 425 bp

Back to Monnina

Pi 0.015

MarkerSeek score 73.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 37
pi: 0.015
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GTGGCCAATAACCCAAGGAA	GTTGTGGCATTAGCGCTTTT	50.0	45.0	58.1	57.9	508-522 bp	1.0	88.6
atpF-atpH_p2	TGGCCAATAACCCAAGGAAA	GTTGTGGCATTAGCGCTTTT	45.0	45.0	57.3	57.9	507-521 bp	1.0	83.6
atpF-atpH_p3	GTGGCCAATAACCCAAGGAA	ATTTATGGGCTGGTTGTGGC	50.0	50.0	58.1	58.8	520-534 bp	1.0	82.7
atpF-atpH_p4	GTGGCCAATAACCCAAGGAA	GGAGGCGGAGGGAAAAATAC	50.0	55.0	58.1	58.3	584-598 bp	0.92	82.6
atpF-atpH_p5	GTGGCCAATAACCCAAGGAA	GAGGCGGAGGGAAAAATACG	50.0	55.0	58.1	58.7	583-597 bp	0.92	79.6