Hotspot

trnS-GCU-trnG-UCC

LSC · 16248-17021 · 774 bp

Back to Monnina

Pi 0.022

MarkerSeek score 75.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 98
pi: 0.022
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGGCGCTTTA	GGGTCAATAGAACGAATCACAC	45.0	45.5	58.0	57.1	651-884 bp	1.0	68.8
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	GGGTCAATAGAACGAATCACAC	50.0	45.5	58.2	57.1	700-933 bp	1.0	67.9
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	GGGTCAATAGAACGAATCACAC	50.0	45.5	57.6	57.1	637-870 bp	1.0	65.8
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGGCGCTTTA	GGGTCAATAGAACGAATCACA	45.0	42.9	58.0	55.7	651-884 bp	1.0	65.3
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	GGGTCAATAGAACGAATCACAC	55.0	45.5	58.5	57.1	632-865 bp	1.0	65.0