Hotspot

psbI-trnS-GCU

LSC · 15990-16159 · 170 bp

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Pi 0.0349

MarkerSeek score 74.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 31
pi: 0.0349
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TCTAACGATCCAGGACGAAA	TAAAGCGCCGGATTGCTAAT	45.0	45.0	56.0	58.0	263-283 bp	1.0	75.8
psbI-trnS-GCU_p2	TCTAACGATCCAGGACGAAA	TAGGGAGAGATGGCTGAGTG	45.0	55.0	56.0	57.9	286-306 bp	1.0	75.4
psbI-trnS-GCU_p3	TCTAACGATCCAGGACGAAA	TTTTTCGTACCGAGGGTTCG	45.0	50.0	56.0	58.2	231-251 bp	1.0	74.3
psbI-trnS-GCU_p4	ATCTAACGATCCAGGACGAA	TAAAGCGCCGGATTGCTAAT	45.0	45.0	55.8	58.0	264-284 bp	1.0	73.9
psbI-trnS-GCU_p5	ATCTAACGATCCAGGACGAA	TAGGGAGAGATGGCTGAGTG	45.0	55.0	55.8	57.9	287-307 bp	1.0	73.6