Hotspot

ndhJ-trnF-GAA

LSC · 12352-12902 · 551 bp

Back to Monnina

Pi 0.0238

MarkerSeek score 73.50

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 551
variable_sites: 65
pi: 0.0238
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TGAAATACAGGATGCTCGTTGA	GGGATAGCTCAGCTGGTAGA	40.9	55.0	57.8	58.0	637-662 bp	0.92	71.2
ndhJ-trnF-GAA_p2	TGAAATACAGGATGCTCGTTGA	CCTCGTGTCACCAGTTCAAA	40.9	50.0	57.8	58.1	601-626 bp	0.92	70.8
ndhJ-trnF-GAA_p3	TGAAATACAGGATGCTCGTTGA	CTGGTAGAGCAGAGGACTGA	40.9	55.0	57.8	57.9	625-650 bp	0.92	70.1
ndhJ-trnF-GAA_p4	TGAAATACAGGATGCTCGTTGA	AGTTCAAATCTGGTTCCCGG	40.9	50.0	57.8	57.8	589-614 bp	0.92	69.6
ndhJ-trnF-GAA_p5	TGAAATACAGGATGCTCGTTGA	ATGGGGATGCATCAGGAATG	40.9	50.0	57.8	57.7	660-685 bp	0.92	68.7