Hotspot

trnN-GUU-trnR-ACG

IRa · 125799-126406 · 608 bp

Back to Monanthes

Pi 0.00658

MarkerSeek score 63.40

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 608
variable_sites: 8
pi: 0.00658
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	ACAGATCTAGTGGAGACGGG	50.0	55.0	58.2	57.9	736 bp	1.0	79.1
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CTCCCGGGAACAGATCTAGT	50.0	55.0	58.2	57.9	745 bp	1.0	79.0
trnN-GUU-trnR-ACG_p3	CGATTGGTCGTAGGTTCGAA	ACAGATCTAGTGGAGACGGG	50.0	55.0	57.7	57.9	740 bp	1.0	78.2
trnN-GUU-trnR-ACG_p4	CGATTGGTCGTAGGTTCGAA	CTCCCGGGAACAGATCTAGT	50.0	55.0	57.7	57.9	749 bp	1.0	78.1
trnN-GUU-trnR-ACG_p5	TTCCTCAGTAGCTCAGTGGT	ACAGATCTAGTGGAGACGGG	50.0	55.0	57.7	57.9	779 bp	1.0	77.9