Hotspot

psbE-petL

LSC · 62664-63668 · 1005 bp

Back to Monanthes

Pi 0.00929

MarkerSeek score 61.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 18
pi: 0.00929
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ATAACCAGCCCGCAATGAAT	CGTATCTTGCTCAGCCCAAT	45.0	50.0	57.9	58.0	1176-1200 bp	1.0	81.9
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.8	58.0	1094-1118 bp	1.0	81.4
psbE-petL_p3	AATAACCAGCCCGCAATGAA	CGTATCTTGCTCAGCCCAAT	45.0	50.0	58.2	58.0	1177-1201 bp	1.0	81.3
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	CGTATCTTGCTCAGCCCAAT	55.0	50.0	58.2	58.0	1089-1113 bp	1.0	81.1
psbE-petL_p5	ACTAACGAATAACCAGCCCG	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.7	58.0	1184-1208 bp	1.0	80.0