Hotspot

petA-psbJ

LSC · 61216-61885 · 670 bp

Back to Monanthes

Pi 0.0159

MarkerSeek score 59.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 21
pi: 0.0159
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATTTTGGCACA	GGTTCCTATTCAGGGTTGGG	42.9	55.0	57.4	57.6	788-838 bp	1.0	66.0
petA-psbJ_p2	TGGCATCTGTTATTTTGGCAC	GGTTCCTATTCAGGGTTGGG	42.9	55.0	57.4	57.6	789-839 bp	1.0	66.0
petA-psbJ_p3	GGCATCTGTTATTTTGGCACA	CAGGGTTGGGTTCATCCTTG	42.9	55.0	57.4	58.5	778-828 bp	1.0	65.9
petA-psbJ_p4	TGGCATCTGTTATTTTGGCAC	CAGGGTTGGGTTCATCCTTG	42.9	55.0	57.4	58.5	779-829 bp	1.0	65.9
petA-psbJ_p5	GGCATCTGTTATTTTGGCACA	ATTCAGGGTTGGGTTCATCC	42.9	50.0	57.4	57.5	781-831 bp	1.0	65.2