Hotspot

ndhC-trnV-UAC

LSC · 49420-50287 · 868 bp

Back to Monanthes

Pi 0.0131

MarkerSeek score 66.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 868
variable_sites: 22
pi: 0.0131
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGAAATGCGACGATCGGAAT	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.8	57.8	978-992 bp	1.0	78.6
ndhC-trnV-UAC_p2	GGGCCTTTACTAGTCGTAGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.5	57.8	1014-1028 bp	1.0	76.3
ndhC-trnV-UAC_p3	CGACGATCGGAATAACGCTT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	971-985 bp	1.0	76.2
ndhC-trnV-UAC_p4	AGAAATGCGACGATCGGAAT	GAAGGTCTACGGTTCGAGTC	45.0	55.0	57.8	57.5	977-991 bp	1.0	75.8
ndhC-trnV-UAC_p5	ACGATCGGAATAACGCTTGA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.4	57.8	969-983 bp	1.0	75.8