Hotspot

trnF-GAA-ndhJ

LSC · 47073-47737 · 665 bp

Back to Monanthes

Pi 0.0155

MarkerSeek score 69.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 17
pi: 0.0155
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GCCTGAAAGTTGGATCGGAT	55.0	50.0	58.0	58.0	781-814 bp	1.0	85.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GCCTGAAAGTTGGATCGGAT	50.0	50.0	58.1	58.0	745-778 bp	1.0	85.0
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	GCCTGAAAGTTGGATCGGAT	55.0	50.0	57.9	58.0	769-802 bp	1.0	84.3
trnF-GAA-ndhJ_p4	GAAAGGATGGTCGGGATAGC	GCCTGAAAGTTGGATCGGAT	55.0	50.0	57.8	58.0	793-826 bp	1.0	84.0
trnF-GAA-ndhJ_p5	TGGAAAGGATGGTCGGGATA	GCCTGAAAGTTGGATCGGAT	50.0	50.0	57.8	58.0	795-828 bp	1.0	83.8