Hotspot

psaA-ycf3

LSC · 41659-42269 · 611 bp

Back to Monanthes

Pi 0.00982

MarkerSeek score 68.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 11
pi: 0.00982
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	788-838 bp	1.0	81.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	717-767 bp	1.0	80.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	790-840 bp	1.0	80.8
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	ATAGCACTTACTCCCGGGAA	50.0	50.0	57.9	57.8	759-809 bp	1.0	80.7
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TAGCACTTACTCCCGGGAAT	50.0	50.0	57.9	57.8	758-808 bp	1.0	80.7