Hotspot

trnC-GCA-petN

LSC · 27622-28429 · 808 bp

Back to Monanthes

Pi 0.017

MarkerSeek score 63.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 25
pi: 0.017
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GTTTCTCTCATTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.0	58.0	923-927 bp	1.0	82.8
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	867-871 bp	1.0	82.4
trnC-GCA-petN_p3	TTCTCTCATTTTGGCGGCAT	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	58.2	58.0	921-925 bp	1.0	81.6
trnC-GCA-petN_p4	GTTTCTCTCATTTTGGCGGC	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.0	57.8	917-921 bp	1.0	81.3
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	861-865 bp	1.0	81.0