Hotspot

atpH-atpI

LSC · 13796-14377 · 582 bp

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Pi 0.0106

MarkerSeek score 61.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 12
pi: 0.0106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCAGCGG	50.0	45.0	58.3	57.9	671-681 bp	1.0	79.2
atpH-atpI_p2	AATAGAAGCAAGTCCGACGG	TTTTGCAACTTTAGCAGCGG	50.0	45.0	57.7	57.9	695-705 bp	1.0	78.9
atpH-atpI_p3	AGTACCCTGACCAACTCCAG	TTTTGCAACTTTAGCAGCGG	55.0	45.0	58.4	57.9	719-729 bp	1.0	78.3
atpH-atpI_p4	AATCCAGCAGCAATAACGGA	TTTTGCAACTTTAGCAGCGG	45.0	45.0	57.6	57.9	673-683 bp	1.0	77.9
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TTTTGCAACTTTAGCAGCGG	45.0	45.0	57.6	57.9	672-682 bp	1.0	77.9